Part:BBa_K4695230

scfL-his device

This device is a fully functional gene capable of expressing ScfL-His fusion protein (BBa_K4695130).

Design Notes

This device uses:

• AB_T7_lacO promoter (BBa_J435350)
• BC_BCD23 RBS (BBa_J435385)
• EF_T7term terminator (BBa_J435361)

All non-coding parts and linkers were chosen from the E. coli Protein Expression Toolkit collection.
This device is compatible with AF vectors.
FLANKED 5' fusion site: GGAG, 3' fusion site: CGCT.

Source

ScfL:
Cellulose binding domain:
Organism: Acetovibrio thermocellus
Structure: https://www.uniprot.org/uniprotkb/Q06851/entry

Cohesin 1:
Organism: Acetovibrio thermocellus
Structure: https://www.rcsb.org/structure/1OHZ

Cohesin 2:
Organism: Acetivibrio cellulyticus
Structure: https://www.rcsb.org/structure/4UYQ

Cohesin 3:
Organism: Bacteroides cellulosolvens
Structure: https://www.rcsb.org/structure/2Y3N

Registry of standard biological parts

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 96
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 781
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 30
  Illegal BamHI site found at 960
  Illegal BamHI site found at 2501
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 96
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 96
  Illegal NgoMIV site found at 1851
  
• 1000
  COMPATIBLE WITH RFC[1000]